Degradation of human cartilage by cytokines in vitro.
نویسندگان
چکیده
منابع مشابه
An In Vitro Model of Cartilage Degradation by Chondrocytes in a Three-Dimensional Culture System
OBJECTIVE Using the alginate bead three-dimensional culturing method, which is considered to be advantageous for the in vitro study of chondrocytes, we confirmed earlier reports concerning the inhibitory effect of TGF-β on IL-1β-induced cartilage destruction and serially evaluated changes in proteinases and their inhibitors in cartilage destruction. METHODS Chondrocytes were cultured on algin...
متن کاملInhibition of interleukin 1 beta induced rat and human cartilage degradation in vitro by the metalloproteinase inhibitor U27391.
Interleukin 1 induced proteoglycan loss from cartilage in vitro was prevented by a biochemical inhibitor of metalloproteinase activity. The inhibitor also partially relieved the inhibition of proteoglycan synthesis caused by interleukin 1. The loss of glycosaminoglycan by rat and human femoral head cartilage in response to human recombinant interleukin 1 beta (rhIL-1 beta) was established, and ...
متن کاملInhibition of interleukin 1 induced rat and human cartilage degradation in vitro by the metalloproteinase inhibitor U 27391
Correspondence to: Dr M P Seed, Departments of Experimental Pathology and Rheumatology, St Bartholomew's Hospital Medical College, Charterhouse Square, London ECIM 6BQ, United Kingdom. Accepted for publication 20 August 1992 Abstract Interleukin 1 induced proteoglycan loss from cartilage in vitro was prevented by a biochemical inhibitor of metalloproteinase activity. The inhibitor also partiall...
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Polymorphonuclear leucocytes (PMNs), which predominate in inflammatory synovial fluid, can degrade cartilage. This was measured by a novel in vitro model; PMNs were incubated for up to one hour with 2 or 3 microns sections of cartilage and the glycosaminoglycan loss determined by microdensitometry after alcian blue staining. Glycosaminoglycan loss could be as a result of damage from reactive ox...
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Cytokines are important regulators of the immune system, and identifying fish cytokines has potential applications for the development of vaccines and/or immunostimulants application in fisheries. In order to understand the immune-related genes triggered by immunostimulants derived from pathogens, we investigated the effects of agonists (lipopolysaccharide (LPS), Poly I:C, and imiquimod) of thr...
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ژورنال
عنوان ژورنال: Annals of the Rheumatic Diseases
سال: 1991
ISSN: 0003-4967
DOI: 10.1136/ard.50.12.967-b